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CHIRALPAK® HSA HPLC Column

CHIRAL-HSA 高效液相色谱柱

品牌
Supelco
货号
58457AST
规格纯度
5 μm particle size, L × I.D. 15 cm × 10 mm
参考价格
200959 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
产品名称:
CHIRALPAK® HSA HPLC Column
CHIRAL-HSA 高效液相色谱柱
产品介绍:

产品说明

一般描述

CHIRALPAK HSA, which uses human serum albumin as the chiral selector, is highly selective for acidic racemates, preferably weak and strong acids, zwitterionic and non-protolytic (neutral) compounds. Phosphate buffers (normally 0.01-0.1M, pH 5-7) with addition of organic modifiers are used as mobile phases. Enantioselectivity and retention can be regulated by changing the mobile phase composition. However, the primary use of CHIRALPAK HSA is for fast drug/protein binding studies (1). To calculate the % protein binding, measure the retention time of an unretained compound (t0) and the compound of interest (tr) on the CHIRALPAK HSA column. Then use the capacity factor equation:
k = (tr - t0)/tr
to calculate the % protein binding (P):
P = 100k/(k+1)

Different types of mobile phases can be used. A mobile phase consisting of 6% 2-propanol in 20 mM potassium phosphate buffer, pH 7.0 gives data in good agreement with literature data. The mobile phase conditions should be chosen to suit the drugs to be tested, i.e., for high protein binding drugs a mobile phase with higher eluting strength might be needed in order to reduce retention times.

(1) Goodman, A.; Gilman, A.G. The Pharmacological Basis of Therapeutics, 9th Edition, McGraw-Hill: New York, 1996; pp 1712-1792.

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法律信息

CHIRALPAK is a registered trademark of Daicel Corp.

基本信息

eCl@ss32110501
NACRESSB.52

产品性质

物料stainless steel column
质量水平100
agencysuitable for USP L79
产品线CHIRALPAK®
包装pkg of 1 ea
manufacturer/tradenameCHIRALPAK®
参数137 bar pressure (2000 psi)
20-30 ℃ temperature
40 ℃ max. temp.
technique(s)HPLC: suitable
长度 × 内径15 cm × 10 mm
基质fully porous particle
基质活性基团human serum albumin phase
粒径5 μm
operating pH2-8
分离技术chiral

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